The Effect of Delayed K3EDTA Blood Samples on Hemoglobin Levels, Erythrocyte, Leukocyte and Platelet Counts with Correction of Peripheral Blood Smears in Referral Samples at Janapria Public Health Center

Siti Zaetun, Rohmi Rohmi, Eni Suryani

Abstract


The results of laboratory examinations greatly influence the diagnosis of the patient's disease. Delay in examining blood samples for hemoglobin levels, leukocyte and platelet counts can allows changes in examination levels and cell morphology. In order for laboratory examination results to be accurate and reliable, there must be control over the pre-analytical, analytical and post-analytical. To determine the effect of delayed K3EDTA blood samples on hemoglobin levels, leukocyte and platelet counts with correction of peripheral blood smears in referral samples at the Janapria Health Center. This study used True Experiment method, 27 experimental units, with pre and post test control design. With data analysis used Anova test. 0 hour, 6 hour, 24 hour delayed experiment on hemoglobin level was 13.9 g/dl; 13.7 g/dl; 14.2 g/dl. Erythrocyte count was 4,87 million cells/µl; 5,84 million cells/µl; dan 5,92 million cells/µl. Leukocytes count was 5,733 cells/µl; 5,789 cells/µl; 5822 cells/µl. Platelet count was 158 thousand/µl; 140.2 thousand/µl; 140.8 thousand/µl. Obtained the probability value was (p) >0.05 on Hb value, leukocyte and platelet count, whereas in erythrocyte count was p < 0,05. There was no significant effect of K3EDTA blood delay on hemoglobin levels, leukocyte and platelet counts with correction of peripheral blood smears, whereas there is signifucant effect in erythrocyte count with correction of peripheral blood smears in referral samples at Janapria Public Health Center.


Keywords


Hemoglobin; Erythrocytes; Leukocytes; Platelets; K3EDTA

Full Text:

PDF

References


Abdurrahman, S., Yayu, S. 2, & Rahayu, S. (2021). Pengaruh Waktu Penyimpanan Serum Pada Pemeriksaan Kolesterol Total. Jurnal MediLab Mandala Waluya, 5(2).

Asiyah, N., Ariyadi, T., & Sukeksi, A. (2018). Perbedaan Jumlah Lekosit Sampel Segera Diperiksa dan Tunda 2 Jam Dan 4 Jam Pada Pasien Lekositosis. Jurnal Universitas Muhamadiyah Semarang, 15–16. http://repository.unimus.ac.id/id/eprint/3182

Balakrishnan, A., Shetty, A., & Vijaya, C. (2018). Estimation of platelet counts: auto analyzer versus counts from peripheral blood smear based on traditional and platelet: red blood cell ratio method. Tropical Journal of Pathology and Microbiology, 4(5), 389–395. https://doi.org/10.17511/jopm.2018.i05.04

Buoro, S., Mecca, T., Seghezzi, M., Manenti, B., Cerutti, L., Dominoni, P., Napolitano, G., Resmini, S., Crippa, A., Ottomano, C., & Lippi, G. (2016). Assessment of blood sample stability for complete blood count using the Sysmex XN-9000 and Mindray BC-6800 analyzers. Revista Brasileira de Hematologia e Hemoterapia, 38(3), 225–239. https://doi.org/10.1016/j.bjhh.2016.05.010

Dameuli, S., Ariyadi, T., & Nuroini, F. (2019). Perbedaan Kadar Hemoglobin menggunakan Hb Meter, Spektrofotometer dan Hematology Analyser pada Sampel Segera dan Ditunda Periksa 20 Jam. Ilmu Kesehatan, 7–17. http://repository.unimus.ac.id

De La Salle, B. J., McTaggart, P. N., Briggs, C., Harrison, P., Doré, C. J., Longair, I., Machin, S. J., & Hyde, K. (2012). The accuracy of platelet counting in thrombocytopenic blood samples distributed by the UK National External Quality Assessment Scheme for General Haematology. American Journal of Clinical Pathology, 137(1), 65–74. https://doi.org/10.1309/AJCP86JMBFUCFCXA

Gandasoebrata, G. (2010). Penuntun Laboratorium Klinik (Cetakan ke). Jakarta: Penerbit Dian Rakyat.

Gunawardena, D., Jayaweera, S., Madhubhashini, G., Lokumarakkala, D. D., & Senanayake, S. J. (2017). Reliability of Parameters of Complete Blood Count With Different Storage Conditions. Journal of Clinical Laboratory Analysis, 31(2). https://doi.org/10.1002/jcla.22042

Hennink, I., van Leeuwen, M. W., Penning, L. C., & Piek, C. J. (2018). Increased number of tissue factor protein expressing thrombocytes in canine idiopathic immune mediated hemolytic anemia. Veterinary Immunology and Immunopathology, 196(November 2017), 22–29. https://doi.org/10.1016/j.vetimm.2017.12.009

Jiwantoro, Diarti, Anggraeni. (2023). Metodologi Penelitian dan Statistik Kesehatan (Untuk Jurusan Teknik Laboratorium Medis). Jakarta: TIM.

Muslim, A. (2017). Pengaruh Waktu Simpan Darah K2EDTA dan Na2EDTA Pada Suhu Kamar Terhadap Kadar Hemoglobin Influence On Storetime of K 2 EDTA and Na 2 EDTA Blood In Room Temperature To Hemoglobin Concentration. Jurnal Analis Kesehatan, 4(2), 392–396.

Nugraha, G., Ningsih, N. A., Sulifah, T., & Fitria, S. (2021). Stabilitas Pemeriksaan Hematologi Rutin Pada Sampel Darah Yang Didiamkan Pada Suhu Ruang Menggunakan Cell-Dyn Ruby. The Journal of Muhammadiyah Medical Laboratory Technologist, 4(1), 21. https://doi.org/10.30651/jmlt.v4i1.8255

Obeidi, N., Safavi, E., & Emami, H. (2020). Evaluation of the effect of temperature and time of incubation on complete blood count ( CBC ) tests. African Journal of Biotechnology, 11(7), 1–4. https://doi.org/10.5897/AJB11.2012

Permenkes RI No 43. (2019). Peraturan Menteri Kesehatan RI No 43 tahun 2019 tentang Pusat Kesehatan Masyarakat. In Kementerian Kesehatan Republik Indonesia: Vol. No. 1335.

Puspitasari, P., Aliviameita, A., Wahyudhi, S. D. Y., & Purnawati, F. P. (2022). Stabilitas Sampel Darah Terhadap Profil Hematologi Dengan Metode Otomatis. The Journal of Muhammadiyah Medical Laboratory Technologist, 5(1), 1. https://doi.org/10.30651/jmlt.v5i1.12667

Rahmnitarini, A., Hernaningsih, Y., & Indrasari, Y. N. (2019). The Stability of Sample Storage for Complete Blood Count (CBC) Toward the Blood Cell Morphology. Bali Medical Journal, 8(2), 391–395. https://doi.org/https://doi.org/10.15562/bmj.v8i2.1369

Ratnasari, D. (2018). Analisis Pelaksanaan Sistem Rujukan Berjenjang Bagi Peserta JKN di Puskesmas X Kota Surabaya. Jurnal Administrasi Kesehatan Indonesia, 5(2), 145. https://doi.org/10.20473/jaki.v5i2.2017.145-154

Saputra, O. D., & Aristoteles, A. (2022). Perbedaan Pemeriksaan Darah Segera Dan Ditunda Selama 6 Jam Pada Suhu 4-8Oc Terhadap Kadar Hemoglobin Dengan Hematology Analyzer. Jurnal ’Aisyiyah Medika, 7(2), 49–56. https://doi.org/10.36729/jam.v7i2.852

Seo, I.-H., & Lee, Y.-J. (2022). Usefulness of Complete Blood Count (CBC) to Assess Cardiovascular and Metabolic Diseases in Clinical Settings: A Comprehensive Literature Review. Biomedicines, 10(11). https://doi.org/10.3390/biomedicines10112697

Sujud, S., Hardiasari, R., & Nuryati, A. (2015). Perbedaan Jumlah Trombosit Pada Darah EDTA Yang Segera Diperiksa dan Penundaan Selama 1 Jam di Laboratorium RSJ Grhasia Yogyakarta. Medical Laboratory Technology Journal, 1(2), 91. https://doi.org/10.31964/mltj.v1i2.21

Tendulkar, A., Jain, P., Gujral, S., Tambe, M., Kenjale, R., & Ganesh, B. (2015). Stability of Selected Hematological Parameters in Stored Blood Samples. Journal of Cell Science & Therapy, 06(05). https://doi.org/10.4172/2157-7013.1000220

Tutik, & Ningsih, S. (2019). Pemeriksaan Kesehatan Hemoglobin di Posyandu Lanjut Usia (Lansia) Pekon Tulung Agung Puskesmas Gadingrejo Pringsewu. Jurnal Pengabdian Farmasi Malahayati, 2(1), 22–26.

Unalli, O. S., & Ozarda, Y. (2021). Stability of hematological analytes during 48 hours storage at three temperatures using Cell-Dyn hematology analyzer. Journal of Medical Biochemistry, 40(3), 252–260. https://doi.org/10.5937/jomb0-27945

Wahyuningsih, F. D. (2017). Perbedaan Jumlah Leukosit Berdasarkan Waktu Pemeriksaan Pada Suhu Kamar Metode Automatic. Poltekkes Kemenkes Semarang.

Zini, G. (2014). Stability of complete blood count parameters with storage: toward defined specifications for different diagnostic applications. In International journal of laboratory hematology (Vol. 36, Issue 2, pp. 111–113). https://doi.org/10.1111/ijlh.12181




DOI: https://doi.org/10.32807/jambs.v12i1.360

Refbacks

  • There are currently no refbacks.


Copyright (c) 2025 Jurnal Analis Medika Biosains (JAMBS)

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 


Jurnal Analis Medika Biosains (JAMBS) Poltekkes Kemenkes Mataram, Jurusan Analis Kesehatan
Jl. Praburangkasari, Dasan Cermen, Sandubaya, Mataram.

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
https://pisangbet.store/ https://pisangbet.cloud/ https://totosuper68.com/ https://www.usaidwildlifeasia.org/ https://yourfoodcareer.com/ https://sbopoker99.com/ https://147.182.141.1/ https://24.199.97.231/ https://joborder.ish.co.id/lol/ https://totokl85.com/ https://thenextreal.net/news/ https://disperindag.pamekasankab.go.id/pacuan/ http://santer.uinbanten.ac.id/ https://totokl98.com/ https://ft.usri.ac.id/ https://regutembakdalam.it.com/ https://wonosobo.pramukajateng.or.id/totokl/ https://referensipropertyindo.com/totokl/ https://prosperengineering.co.th/ https://assethot.com/ https://siarvi.kemkes.go.id/ http://semnas.radenfatah.ac.id/ https://bukaantotokl.com/ https://www.wifeemade.com/ https://adirach.com/ https://heylink.me/totokudalari/ https://heylink.me/totosuper-login/ https://umart.um.ac.id/ https://cakrajb.um.ac.id/ http://absen.bbpomserang.com/ https://lptqkaltim.or.id/ https://ejurnal.unim.ac.id/ https://bisnisdigital.darmajaya.ac.id/ https://pertanian.jatimprov.go.id/ https://manumminen.com/ https://artandsignature.com/ https://gis-bpkad.blitarkota.com/ https://esteghlalshop.com/ https://fightthebull.com/