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Email this article Methicillin–resistant Staphylococcus aureus (MRSA) Identification Among Health Care Workers in the Intensive Care of Nusa Tenggara Barat Regional General Hospital
Abstract
Healthcare workers (HCW’s) are the primary providers of services in healthcare facilities. The high frequency of contact between healthcare workers and patients renders them susceptible to the transmission of MRSA. This research was conducted to detect the presence of the MRSA using Mec A gene and their sensitivity with antibiotics among HCWs in the intensive care of the Nusa Tenggara Barat Regional General Hospital. This is cross-sectional study with purposive random sampling nostril swabs and information about daily routines of changing outfit HCW’s ICU, HCU, PICU rooms between January-March 2024. The examination included a microbiological examination and a PCR of the Mec A gene.Microbiological examination of 45 HCWs nostril swabs revealed the presence of 31 isolates of S.aureus, 25 of isolate were identified as Methicillin-Susceptible Staphylococcus aureus (MSSA), and 6 as MRSA. Four MRSA isolates from the HCW in ICU exhibited the highest resistant to Cefoxitin (75%), while two HCW’s PICU isolates demonstrated 100% resistant to Amoxicillin. The six MRSA isolates were confirmed positive for the Mec A gene by PCR examination using the Mec A primer at 532 bp, and three of them is a HCW’s who’s didn’t change clothes every day. The presence of the MRSA among HCWs in the intensive care unit of Nusa Tenggara Barat Regional General Hospital was 19%. Furthermore, it was found that all of S.aureus were still sensitif to vancomycin.
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Alanazi, F. K. (2023). Healthcare-associated infections in adult intensive care units: A multisource study examining nurses’ safety attitudes, quality of care, missed care, and nurse staffing. Intensive Crit Care Nurs. https://doi.org/DOI: 10.1016/j.iccn.2023.103480
Algammal, A. M., Hetta, H. F., Elkelish, A., Alkhalifah, D. H. H., Hozzein, W. N., Batiha, G. E. S., Nahhas, N. El, & Mabrok, M. A. (2020). Methicillin-resistant staphylococcus aureus (MRSA): One health perspective approach to the bacterium epidemiology, virulence factors, antibiotic-resistant, and zoonotic impact. Infection and Drug Resistant, 13, 3255–3265. https://doi.org/10.2147/IDR.S272733
Blot, S., Ruppé, E., Harbarth, S., Asehnoune, K., Poulakou, G., Luyt, C.-E., Rello, J., Klompas, M., Depuydt, P., Eckmann, C., Martin-Loeches, I., Povoa, P., Bouadma, L., Timsit, J.-F., & Zahar, J.-R. (2022). Healthcare-associated infections in adult intensive care unit patients: Changes in epidemiology, diagnosis, prevention and contributions of new technologies. Intensive and Critical Care Nursing, 70, 103227. https://doi.org/10.1016/j.iccn.2022.103227
Duong, T. B., Duong, M. C., Campbell, J. I., Nguyen, H. V. M., Nguyen, H. H., Bui, H. T. B., Nguyen, C. V. V., & Heywood, A. (2022). MRSA carriage among healthcare workers in a Vietnamese intensive care unit: a prospective cohort study. Drug Target Insights, 16(1), 71–77. https://doi.org/10.33393/dti.2022.2504
Erlin, E., Rahmat, A., Redjeki, S., & Purwianingsih, W. (2020). Deteksi Methicilin Resistant Staphylococcus aureus ( MRSA ) Sebagai Penyebab Infeksi Nosokomial Pada Alat-Alat di Ruang Perawatan Bedah. Quagga: Jurnal Pendidikan Dan Biologi, 12(2), 137. https://doi.org/10.25134/quagga.v12i2.2671
Gehrke, A. K. E., Giai, C., & Gómez, M. I. (2023). Staphylococcus aureus Adaptation to the Skin in Health and Persistent/Recurrent Infections. In Antibiotics (Vol. 12, Issue 10). Multidisciplinary Digital Publishing Institute (MDPI). https://doi.org/10.3390/antibiotics12101520
Khairullah, A. R., Kurniawan, S. C., Silaen, O. S. M., Effendi, M. H., Sudjarwo, S. A., Ramandinianto, S. C., Gelolodo, M. A., Widodo, A., Riwu, K. H. P., Kurniawati, D. A., & Rehmank, S. (2023). Methicillin Resistant Staphylococcus aureus (MRSA) Isolation and mecA Gene Detection from Milk and Farmer Hand Swab in Tulungagung, Indonesia. Tropical Animal Science Journal, 46(2), 231–238. https://doi.org/10.5398/tasj.2023.46.2.231
Lade, H., & Kim, J. S. (2023). Molecular Determinants of β-Lactam Resistant in Methicillin-Resistant Staphylococcus aureus (MRSA): An Updated Review. In Antibiotics (Vol. 12, Issue 9). Multidisciplinary Digital Publishing Institute (MDPI). https://doi.org/10.3390/antibiotics12091362
Laux, C., Peschel, A., & Krismer, B. (2019). Staphylococcus aureus Colonization of the Human Nose and Interaction with Other Microbiome Members . Microbiology Spectrum, 7(2). https://doi.org/10.1128/microbiolspec.gpp3-0029-2018
Liu, J. (2016). Staphylococcal chromosomal cassettes mec (SCCmec): A mobile genetic element in methicillin-resistant Staphylococcus aureus. Microbial Pathogenesis, Volume 101, Pages 56-67. https://doi.org/DOI: 10.1016/j.micpath.2016.10.028
Popovich, K. J., Green, S. J., Okamoto, K., Rhee, Y., Hayden, M. K., Schoeny, M., Snitkin, E. S., & Weinstein, R. A. (2021). MRSA Transmission in Intensive Care Units: Genomic Analysis of Patients, Their Environments, and Healthcare Workers. Clinical Infectious Diseases, 72(11), 1879–1887. https://doi.org/10.1093/cid/ciaa731
DOI: https://doi.org/10.32807/jambs.v12i1.441
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