Bio Formula – BS effervescent (Bio- Bacillus sphaericus) is an Effervescent powder-shaped formula that is easy to use by the public. Lombok's local effervescent bio-BS isolated formula has a weakness in terms of smell. The results from the literature search that fragrant lemongrass has a distinctive smell and can kill Aedes aeygpti, but scientific data has not been obtained about the ability of fragrant lemongrass to kill Anopheles Sp in the form of larvae and mosquitoes. The study aimed to find out the effect of the combination formulation of Bio-BS Effervescent local isolate Lombok Island with the addition of lemongrass powder for viability and the amount of toxin crystal protein Bacillus sphaericus. This research method is exploratory and experimental in the laboratory with the design of Post Test Only Control Group Design. The study used six treatment formulations. Samples of Anopheles sp larvae research in Batu Layar lagoon, West Lombok regency and pelur lagoon in Peringgesela, East Lombok regency and from the results of colonization of larvae. The independent Variable is a combination formulation of Bio-BS Effervescent isolated locally from Lombok Island with the addition of fragrant lemongrass powder. The dependent variable is the mortality of Larvae Anophels Sp, Viability of B. Sphaericus, and Amount of Toxin Protein Production of B. Sphaericus. The larvae death rate, the concentration of cells/endospores, and the number of repeats in each container are then tabulated and analyzed using Probit Analysis with the help of MINITAB 16 software. B. sphaericus viability data and the amount of endospore toxin protein crystal production were descriptively analyzed.

Aji, R. (2017). Pengaruh Serai Wangi Terhadap Keberadaan Larva Aedes Aegypti Pada Tempat Penampungan Air. Jurnal Vokasi Kesehatan. http://ejournal.poltekkes-pontianak.ac.id/index.php/JVK 3 (1)

Arifin, M. N. (2014). Pengaruh Ekstrak n-Heksan Serai Wangi (Cymbopogon nardus (L.) Randle) pada berbagai konsentrasi terhadap periode menghisap darah dari nyamuk Aedes aegypti.

Berry, C., Hindley, J., Ehrhardt, A. F., Grounds, T., & de Souza, E. (1993). Genetic determinants of host ranges of Bacillus sphaericus larvacidal toxins. J. Bacteriol, 175, 510–518.

Charles, J. F., Kalfon, A., Bourgouin, C., & de Barjac, H. (1988). Bacillus sphaericus asporogenous mutants: ultrastructure, mosquito larvacidal activity and protein analysis. Ann. Inst. Pasteur/Microbiol, 139, 243–259.

Dulmage, T., Yousten, A. A., Singer, S., & Lacey, L. A. (1990). Guidelines for Production of Bacillus Thuringiensis H-14 and Bacillus Sphaerirus. WHO Special Programme for Reasearch and Training in Tropical Disease (TDR).

Fikri, Z., & Jiwintarum, Y. (2016). Media Kultur Sederhana Untuk Pembiakkan Bacillus Sphaericus Isolat Lokal Pulau Lombok Untuk Pengendalian Larva Nyamuk Anopheles Sp. Laporan Pengembangan Penelitian Dosen Skema Dosen Pemula Politeknik Kesehatan Mataram Kemenkes RI.

Mirawati, P., Simaremare, E. S., & Pratiwi, R. D. (2018). Uji Efektivitas Repellent Sediaan Lotion Kombinasi Minyak Atsiri Daun Zodia (Evodia suaveolens Scheff) dan Minyak Atsiri Batang Serai (Cymbopogon citratus) Terhadap Nyamuk Aedes aegypti L. PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia), 15(1), 1. https://doi.org/10.30595/pharmacy.v15i1.2286

Riris, M., Umar, M., & Fitriani, J. (2019). Uji Efektivitas Repellent Minyak Atsiri Daun Serai Wangi Cymbopogon nardus (L) Randle Yang Dikombinasi Dengan Minyak Atsiri Daun Kayu Putih (Melaleuca leucadendron Linn.) Dan VCO (Virgin Coconut Oil) Terhadap Nyamuk Aedes aegypti. Medika Tadulako, Jurnal Ilmiah Kedokteran, 6(1 Januari 2019).

Satriawan, A. (2014). Efektivitas Serai Dapur (Cyambopogon citrus) sebagai larvasida pada larva nyamuk Aedes Sp Instar III/IV. Fakultas Kedokteran Syariff Hidayatullah Jakarta.

Support., M. 17. (2015). Probit Analysis. http://support.minitab.com/en-us/minitab/17/topic-library/modeling-statistics/reliability/types-of-reliability-analyses/probit-analysis/

Susilawati, L., & Susiawati Purnomo, E. (2016). Viabilitas Sel Bakteri Dengan Cryoprotectant Agents Berbeda (Sebagai Acuan Dalam Preservasi Culture Collection di Laboratorium Mikrobiologi). Biogenesis: Jurnal Ilmiah Biologi, 4(1), 34–40. https://doi.org/10.24252/bio.v4i1.1118

Thiery, I., & de Barjac, H. (1989). Selection of the most potent Bacillus sphaericus strains, based on activity ratios determined on three mosquito species. App. Microbiol. Biotechnol, 31, 577–581.