Correlation The Number Of Erythrocytes And Glucose Level From Serum Which 2 Hours Delayed From Delayed

Bastian Darwin, Eka Yuniar, Endang Endang, Ian Kurniawan

Abstract


In the processing of specimens, serum specifically should be separated from blood cell the process of glucose glycolysis which caused decreasing levels of glucose. The very high number of erythrocyte can cause a glycolysis, so the decreasing of the levels of glucose will be happen. This study was pre-experiment using One Group Pretest Posttest. The sample was taken using purposive sampling technique and there were 30 subjects. The sample was EDTA bloods and serum which had been treated such as Separation of Glucose Serum which was 2 hours delayed and Separation of Glucose Serum which was checked directly. From this study we can found that the average of the glucose levels on serum separation which is 2 hours delayed was 91 mg/dL and the Separation of Glucose Serum which is checked directly was 97mg/dL. Based on paired T test the result, the data showed that there were differences from average levels of glucose between serum which was checked directly and separation of Serum which was 2 hours delayed. The average from the number of erythrocyte was 5,0 million/µL. Based on pearson correlation, there was no correlation between the number of erythrocyte and glucose level on serum separation which is 2 hours delayed.

Keywords


The number of Erythrocyte, The level of Glucose, Pre-analytical.

Full Text:

PDF

References


Bruns DE & Knowler W (2009). Stabilizatin of Glucose in Blood Samples : Why It Matters. Clinical Chemistry : 55 (5) 850– 852. Diaskes November 2015.

Champe PC, Harvey RA & Ferrier DR (2010). Biokimia Ulasan Bergambar. Jakarta: EGC, pp : 116-118.

Datta RR, Baruah A, Pathak MS, Barman M, Borah MB (2014). Effect of Temperature and Serum-Clot Contact Time on the Clinical Chemistry. ISSN : Indian journal of Basic and Applied Medical Research, 4 (1) 356 – 362. Diaskes November 2015.

Heijnen HFG, Oorschot V, Sixma JJ, Slot JW & James DE (1997). Trombin Stimulates Glucose Transport in Human Platelets Via the Translocation of the from α-Granules to the Cell. The Journal of Cell Biology, Vol :138 (323-330). Diaskes Juni 2016.

Korgun DK, Sarikaoglu SB, Altuntas H, Demir R & Korgun ET (2009). Type-2 Diabetes Down-Regulates Glucose Transporter Proteins and Genes of the Human Blood Leukocytes. Informa Healti Care : The Scandinavian Journal of Clinical & Laboratory Investigation. Vol 69 (3) Hal : 350-358. Diaskes Juni 2016.

Medical Laboratory (2008). Preanalytic Laboratory Errors : Identification and Prevention. Mayo Clinical : Vol 33 No.12. Diaskes Novemebr 2015.

Murray RK, Granner Daryl K, Mayes Peter A & Rodwell Victor W (2003). Biokimia Harper. Edisike 25. Jakarta: EGC, pp : 729-731.

Nwosu, O. K & Nwani, C. D (2008). Stability of Serum/Plasma Glucose for the Diagnosis of Diabetes Mellitus. ISSN :Bio-Research, 6 (2) 380 – 383. Diaskes November 2015.

Oddoze Christiane, Lombard Elise & Portugal Henri (2012). Stability study of 81 analytes in human whole blood, in serum and in plasma. ELSEVIER : Clinical Biochemistry (45) 464 – 469.Diaskes November 2015.

Sacher RA & McPherson RA (2004). Tinjauan Klinis Hasil Pemeriksaan Laboratorium. Jakarta: EGC, p : 288. Sadikin H.M (2001). Biokimia Darah.Jakarta: Widya Medika, pp : 12-15.




DOI: https://doi.org/10.32807/jambs.v7i1.164

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Jurnal Analis Medika Biosains (JAMBS)

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 


Jurnal Analis Medika Biosains (JAMBS) Poltekkes Kemenkes Mataram, Jurusan Analis Kesehatan
Jl. Praburangkasari, Dasan Cermen, Sandubaya, Mataram.

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.