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Modifikasi Bak Pengecatan pada Pembuatan Sediaan Apusan Darah Tepi

Miftahul Jannah, Affan Ardiyanto

Abstract


Pembuatan preparat apusan darah tepi menjadi suatu keterampilan yang harus dimiliki oleh seorang tenaga laboratorium kesehatan. Sediaan apusan darah tepi merupakan bagian yang penting dari rangkaian pemeriksaan hematologi. Proses mengering-anginkan ini merupakan bagian penting yang dilakukan sebelum sediaan apusan darah tepi difiksasi dengan metil alkohol danĀ  bagian penting setelah selesai proses pengecatan. Lamanya waktu yang dibutuhkan untuk proses kering-anginkan sediaan apusan darah ini yang menjadi latar belakang membuat modifikasi bak pengecatan pada pembuatan sediaan apusan darah.Tujuan penelitian ialah melakukan modifikasi terhadap bak pengecatan pada pembuatan preparat apusan darah tepi dan mengefisienkan waktu pembuatan preparat apusan darah. Penelitian ini merupakan penelitian eksperimen dengan memodifikasi bak pengecatan yang terbuat dari bahan akrilik berukuran 30cm x 10cm x 10cm yang ditambahkan kipas mini berukuran 4 cmx 4cm x1 cm. Untuk menghidupkan kipas mini digunakan power bank dengan pengatur kecepatan. Dilakukan uji fungsi dan uji efektifitas waktu pengeringan. Pada bak pengecatan standar rata-rata waktu pengeringan sediaan apusan darah ialah 6 menit 5 detik, bak pengecatan modifikasi dengan kecepatan 1000 rpm ialah 4 menit 32 detik, kecepatan 2000 rpm ialah 2 menit 14 detik,kecepatan 3000 rpm ialah 1 menit 58 detik dan kecepatan 4000 rpm ialah 1 menit 28 detik. Analisis data dilakukan dengan uji normalitas dan uji bivariat pada perangkat lunak SPSS versi 19. Hasil analisis uji normalitas metode Shapiro-Wilk menunjukkan data tidak berdistribusi normal dan uji bivariat metode Mann-Whitney menunjukkan terdapat perbedaan bermakna waktu pengeringan sediaan apusan darah tepi menggunakan alat modifikasi bak pengecatan sediaan apusan darah tepi. Untuk uji kualitas gambaran sel darah merah dan sel darah putih memiliki kualitas sama menggunakan bak pengecatan standar dengan modifikasi bak pengecatan pada pembuatan sediaan apusan darah tepi.


Keywords


modifikasi bak pengecatan; sediaan apusan darah tepi

Full Text:

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References


Gandasoebrata R. (2013). Penuntun Laboratorium Klinis.Dian Rakyat. Jakarta

Kiswari Rukman. (2014). Hematologi & Transfusi. Erlangga. Jakarta.

Notoatmodjo,Soekidjo. (2010). Metodologi Penelitian Kesehatan. EGC. Jakarta

Nugraha,Gilang. (2017). Panduan Pemeriksaan Laboratorium Hematologi Dasar Edisi 2. Trans Info Media. Jakarta

Riswanto. (2013). Pemeriksaan Laboratorium Hematologi. Alfamedia dan Kanal Medika.Yogyakarta

Rodak, B.F,George A,F dan Kathryn D. (2007). Hematology: Clinical Principles and Applications.Sanders Elsevier.USA




DOI: https://doi.org/10.32807/jambs.v9i2.265

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