Quantity Evaluation of Laactic Acid Bacteria Using the Fncc 0027 and C410li Gene on Different Storage of Acid Bamboo

Putri Widelia Welkriana, Halimatussa'diah Halimatussa'diah

Abstract


The fiber content in bamboo shoots is higher than other tropical vegetables. The water content in saw bamboo shoots is quite high, causing the bamboo shoots to have a shelf life of only 2 days. The fermentation process is characterized by the conversion of carbohydrates into lactic acid by bacteria. This study aimed to calculate LAB with FNCC 0027 and C410LI genes in tamarind shoots with different storage periods. The method used in this study is a descriptive method to describe the total plate number of microbes, LAB examination and molecular identification of bacteria found in tamarind shoots with different storage times. The results showed that all samples showed the presence of microbial TPC, examination. As for molecular identification, it was found that samples 1, 2, and 3 were identified as LAB with the C4101LI gene, while the FNCC0027 gene was not detected. Concluded that the total plate number values with different storage times of tamarind shoots, namely 0, 24, and 48 hours were 2.5x105 CFU/g, 5.4x105 CFU/g, and 7.3x105 CFU/g. In samples 1, 2, and 3, there were lactic acid bacteria of the type Bacillus plantarum C401L1.


Keywords


Acid shoots; storage time; Lactic acid bacteria;

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References


Adesetan, T.O., Efuntoye, M.O. and Babalola, O.O. (2020). Genotypic Profiling of Bacillus cereus Recovered From Some Retail Foods in Ogun State, Nigeria, and Their Phylogenetic Relationship. International Jour. of Microbial. //doi.org/10.1155/2020/3750948.

Apridani, E. 2013. Viabilitas Lactobacillus plantarum 1 Yang Diisolasi Dari Susu Kedelai Terfermentasi Spontan Terhadap Asam Klorida Dan Garam Empedu. Skripsi. Universitas Riau Pekanbaru. Riau.

Chiu, Y.H., Gaskins, A.J., Williams, P.L., Mendiola, J., Jørgensen, N. and Levine, H. (2016). Intake of Fruits and Vegetables with Low-to-moderate Pesticide Residues is Positively Associated with Semen-Quality Parameters Among Young Healthy Men. Journal nutrition (146). pp:1084–1092. doi: 10.3945/jn.115.226563

Handoko, A., 2003. Budidaya Bambu Rebung. Kanisius. Yogyakarta.

Hou, L. and Majumder, E.L.W. (2021). Potential For and Distribution of Enzymatic Biodegradation of Polystyrene by Environmental Microorganisms. Materials. Vol. 14(3). pp:503.

Marchlewicz, A., Domaradzka, D., Guzik, U., Wojcieszyńska, D. (2016). Bacillus plantarum B1 (2015b) is a Gram-Positive Bacteria Able to Degrade Naproxen and Ibuprofen. Water, Air, & Soil Pollution. Vol. 227(6). pp: 1-8

Rostini, I. 2007. Peranan Bakteri Asam Laktat (Lactobacillus plantarum) terhadap Masa Simpan Fillet Nila Merah pada Suhu Rendah. www.scribd.com/meexchild/d/19097711-Peranan-Bakteri-Asam. [akses tanggal 18 Maret 2022].

Sambrook, J. and Russell, D.W. (2001). Molecular Cloning a Laboratory Manual. 3rd ed. New York. Cold Spring Harbor Laboratory Pr.

Wei, S., Chelliah, R., Park, B. J., Kim, S. H., Forghani, F., Cho, M. S. and Oh, D. H. (2019). Differentiation of Bacillus plantarum from Bacillus cereus group using a unique marker based on real-time PCR. Frontiers in microbiology. Vol. 10:883.




DOI: https://doi.org/10.32807/jambs.v10i1.296

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